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Detection and Speciation of Mycoplasma spp. in Bulk Milk Tanks

Mastitis caused by Mycoplasma spp. is economically devastating to the U.S. dairy industry. The contagious nature of pathenogenic Mycoplasma spp., including M. bovis, makes early detection a key component of maintaining herd health. Bulk tank milk sampling is a common method of monitoring herd health, but current testing methods offer limited information regarding the actual species of Mycoplasma causing a particular infection. The combined economic loss and limited testing for speciation of Mycoplasmas provides an opportunity for a technology that provides information down to the level of the particular species of Mycoplasma causing an infection. Utah State University is seeking a partner to exploit the commercial opportunities for a novel, patent pending diagnostic method for the detection and speciation of Mycoplasma spp., which was developed by one of USU’s veterinarian microbiologists. In addition to detecting and speciating Mycoplasma spp. in bulk milk tanks, the method can also be applied to virtually any testing situation where speciation of Mycoplasmas is desired. If you are interested in learning more about this exciting new diagnostic technique, please contact Utah State University’s Technology Commercialization Office.
Features and Benefits
  • Bulk tank milk testing
  • Herd monitoring
  • Cell culture
  • Veterinary diagnostics
  • Infectious disease research
  • Innovative real-time PCR method, allowing for accurate speciation of Mycoplasma spp.
  • Single PCR reaction can identify multiple Mycoplasma spp., saving time and money
This patent pending technology uses real-time PCR and dissociation curve analysis for detection and speciation of Mycoplasma spp. Primers used amplify a conserved region of the rRNA intergenic spacer region of the Mycoplasma genus. By determining the dissociation temperature of the double-stranded DNA generated from the PCR reaction, the species of mycoplasma can determined. Optional post-PCR analysis provides for further ability to distinguish new or novel Mycoplasma spp.
Development Stage
This technology has been demonstrated to work on actual bulk tank milk samples. In addition to M. bovis, 4 other species of Mycoplasmas, M. alkalescens, M. arginini, M. bovigenitalium, and M. gateae, were identified in bulk tank milk samples.
U.S. patent pending
Joe Christison
Senior Commercialization Associate
Life Sciences
(435) 797-9614
Reference: W09077